Table 1 Sequences of adaptors and primers used for pre-amplification and selective amplification in AFLP and MSAP analysis
Adaptors/primers | Sequence (5′–3′) |
|---|---|
Mse I adaptor-1 | GACGATGAGTCCTGAG |
Mse I adaptor-2 | TACTCAGGACTCAT |
Eco RI adaptor-1 | CTCGTAGACTGCGTACC |
Eco RI adaptor-2 | AATTGGTACGCAGTCTAC |
Hpa II/ Msp I adaptor-1 | GATCATGAGTCCTGCT |
Hpa II/ Msp I adaptor-2 | CGAGCAGGACTCATGA |
Eco RI pre-selective primer | GACTGCGTACCAATTCA |
Mse I pre-selective primer | GATGAGTCCTGAGTAAC |
Hpa II/ Msp I pre-selective primer | ATCATGAGTCCTGCTCGG |
Eco RI selective primer-2 | GACTGCGTACCAATTCAAG |
Eco RI selective primer-3 | GACTGCGTACCAATTCACA |
Eco RI selective primer-4 | GACTGCGTACCAATTCACT |
Eco RI selective primer-5 | GACTGCGTACCAATTCACC |
Eco RI selective primer-6 | GACTGCGTACCAATTCACG |
Eco RI selective primer-7 | GACTGCGTACCAATTCAGC |
Eco RI selective primer-8 | GACTGCGTACCAATTCAGG |
Mse I selective primer-2 | GATGAGTCCTGAGTAACAC |
Mse I selective primer-3 | GATGAGTCCTGAGTAACAG |
Mse I selective primer-5 | GATGAGTCCTGAGTAACTA |
Mse I selective primer-6 | GATGAGTCCTGAGTAACTC |
Mse I selective primer-7 | GATGAGTCCTGAGTAACTG |
Mse I selective primer-8 | GATGAGTCCTGAGTAACTT |
Hpa II/ Msp I selective primer-1 | ATCATGAGTCCTGCTCGGTAA |
Hpa II/ Msp I selective primer-2 | ATCATGAGTCCTGCTCGGTCC |
Hpa II/ Msp I selective primer-3 | ATCATGAGTCCTGCTCGGTTC |
Hpa II/ Msp I selective primer-6 | ATCATGAGTCCTGCTCGGTAG |
Hpa II/ Msp I selective primer-7 | ATCATGAGTCCTGCTCGGTTG |
Hpa II/ Msp I selective primer-8 | ATCATGAGTCCTGCTCGGTCA |