Figure 1

Summary of bioinformatics processes. The RNA-seq experiment produced a collection of 382,933 fragments that aggregated 47.5% of the LCM-aRNA reads. The annotation strategy assigned 73% of the mapped reads. A total of 221,716 genomic fragments remain unannotated. A total of 86.8% of the annotated reads are located in stop codon or 3′UTR regions, whereas only 5.5% are located in exons, 1.2% in start codon/ 5′UTR regions and 6.5% in introns. The final dataset conserved a single fragment per gene corresponding to the nearest 3′UTR region with the highest number of reads. This dataset aggregated 89.4% of the annotated LCM-aRNA reads (86.8% were located in 3′ UTR regions and 2.6% were located in exons).