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Table 2 List of repressed genes in intra-colonic environment compared to spent medium with RT-qPCR confirmation

From: A unique in vivo experimental approach reveals metabolic adaptation of the probiotic Propionibacterium freudenreichii to the colon environment

Locus tag

Gene

Descript

Function

FC microarray (induced in colon)

pvalue microarray

FC RT-qPCR (induced in colon)

pvalue RT- qPCR

PFREUD_14940

sufS

Cysteine desulphurases, SufS

1.2 Transport/binding proteins and lipoproteins

7,4

2,5E-07

1,4

6,9E-02

PFREUD_19650

feoB

Ferrous iron uptake protein B 9.A.8.1.x

1.2.3 Transport/binding of inorganic ions

6,7

1,0E-07

1,3

3,5E-02

PFREUD_20460

cycA1

D-serine/D-alanine/glycine transporter 2.A.3.1.7

1.2.5 Transport/binding of amino-acids

16,5

3,9E-05

2,0

2,4E-02

PFREUD_05220

nuoG

NADH-quinone oxidoreductase chain G

1.4 Membrane bioenergetics (electron transport chain and ATP synthase)

9,9

4,3E-08

5,3

6,0E-03

PFREUD_10590

mcoE

Methylmalonyl-CoA epimerase

2.1.1 Specific carbohydrate metabolic pathway

9,3

3,0E-07

21,7

6,8E-02

PFREUD_01040

gntK

Gluconate kinase (Gluconokinase)

2.1.1 Specific carbohydrate metabolic pathway

2,9

2,9E-05

39,5

4,3E-02

PFREUD_18870

 

Methylmalonyl-CoA carboxytransferase 5S subunit.

2.1.1 Specific carbohydrate metabolic pathway

10,3

1,1E-07

10,0

1,3E-02

PFREUD_17320

eno1

Enolase 1

2.1.2 Main glycolytic pathways

8,4

3,6E-07

26,3

2,0E-03

PFREUD_23890

fba2

Fructose-bisphosphate aldolase class I

2.1.2 Main glycolytic pathways

21,3

1,1E-08

68,6

2,0E-03

PFREUD_05650

tuf

Elongation factor Tu

3.7.4 Translation elongation

4,8

4,8E-05

2,0

4,9E-01

PFREUD_17840

dnaK1

Chaperone protein dnaK 1

3.9 Protein folding

15,5

1,5E-07

23,3

2,5E-03

PFREUD_19250

clpB 1

Chaperone clpB1

3.9 Protein folding

3,6

4,1E-04

10,9

6,6E-02

PFREUD_22780

hsp20 1

Heat shock protein 20 1 (20 kDa chaperone 1)

3.9 Protein folding

36,6

2,5E-08

20,2

1,4E-02