Male infertility and copy number variants (CNVs) in the dog: a two-pronged approach using Computer Assisted Sperm Analysis (CASA) and Fluorescent In Situ Hybridization (FISH)

Table 2 Fluorescence In Situ Hybridization results on sperm cells (S-FISH) and lymphocytes from 6 samples

		#1 Cane Corso (Xanto)			#2 Dogo Argentino (Tiburon)			#5 Cane Corso (Godzilla)			#7 Cane Corso (Master)			#11 Cane Corso (Agrado)			#12 Cane Corso (Sam)
		Lymphocytes	Sperm		Lymphocytes	Sperm		Lymphocytes	Sperm		Lymphocytes	Sperm		Lymphocytes	Sperm		Lymphocytes	Sperm
Gene	BAC	Dup status	CN(s)	Dup status	Dup status	CN(s)	Dup status	Dup status	CN(s)	Dup status	Dup status	CN(s)	Dup status	Dup status	CN(s)	Dup status	Dup status	CN(s)	Dup status
DNM2	CH82-321J09	Dup	1.93	Dup	Dup	1.83	Dup	Dup (Het)	1.5	Dup (Het)	Dup	2	Dup	Dup	1.81	Dup	Sin	-	-
SOX8	CH82-324I01	Sin	1.36	Sin	Sin	1.45	Sin	Sin	1.15	Sin	Sin	1.42	Sin	Dup	1.69	Dup	Sin	-	-
TEKT1	CH82-509B23	Dup	1.71	Dup	Dup	2	Dup	Dup	1.63	Dup	Dup	1.68	Dup	Dup	1.95	Dup	Sin	-	-
DBY	CH82-297B18	Sin	1	Sin	Sin	1	Sin	Sin	1.00	Sin	Dup	1.78	Dup	Sin	1	Sin	Sin	-	-
USP9Y	CH82-53P08	Sin	1.31	Sin	Sin	1.21	Sin	Dup	1.67	Dup	Sin	1	Sin	Dup	1.76	Dup	Dup	-	-

BAC CH82-297B18 was used as a single-copy control for all samples except for case #7, where it showed a duplication pattern. In this particular case we used BAC CH82-53P08 for this purpose. Sin, Single; Dup, Duplicated; CN, copy number; Het, Duplication in Heterozygosis.

ISSN: 1471-2164