Figure 4

The karyotype and ideograph for C. lacryma-jobi mitotic metaphase chromosomes. The karyotype and ideograph for C. lacryma-jobi mitotic metaphase chromosomes. (a) Mitotic metaphase chromosomes were counterstained with DAPI and pseudocolored in red and chromosomes are numbered according to our karyotyping analysis; (b) FISH with the probe cocktail of SatS5 (green), 5S (red) and 45S (yellow); (c) The same spread was reprobed with the probe cocktail of CentX (green) and SatS4 (red); (d) The same spread was reprobed with the probe cocktail of SatS3 (green) and SatS4 (red); (e) Individual chromosomes displayed according to the results in (a-d); (f) The Ideograph showing the position and intensity of CentX(blue), SatS3 (light-blue), SatS4 (red), SatS5 (green), 45S rDNA (yellow) and 5S rDNA (purple), bar = 5 μm.