Figure 5From: Differential genome evolution and speciation of Coix lacryma-jobi L. and Coix aquatica Roxb. hybrid guangxi revealed by repetitive sequence analysis and fine karyotypingThe karyotype for C. aquatica HG mitotic metaphase chromosomes. The karyotype for C. aquatica HG mitotic metaphase chromosomes. (a) The mitotic metaphase chromosomes (numbered from 1 to 10 ) were counterstained with DAPI and pseudocolored in red; (b) FISH with the probe cocktail of SatS3 (green) and SatS2 (red); (c) A merged imagine with CentX in green and SatS2 in red; (d) The same spread was reprobed with the probe cocktail of SatS5 (green), 5S (red) and 45S (yellow); (e) The same spread was reprobed with the probe cocktails of SatS1 (green) and SatS2 (red); (f) The same spread was reprobed with the probe cocktail of CentX (green) and C. lacryma-jobi gDNA (red); (g) The twenty chromosomes closely homologous to C. lacryma-jobi were separated from figure (a-g) and listed in numerical order; (h) Ten chromosomes distant to C. lacryma-jobi were separated from figure (a-g) and listed in numerical order, bar = 5 μm.Back to article page