Figure 6

FISH analysis of asymmetric satellite distribution on pachytene chromosomes of C. aquatica HG. FISH analysis of asymmetric satellite distribution on pachytene chromosomes of C. aquatica HG. The arrowhead pointed FISH signals, and the hollow arrowhead pointed the absence of the signals from the same type of probes or the obvious weaker signals. (a) Satellite staining on a pachytene chromosome of C. aquatica HG with SatS3 (green) and SatS5 (red) probes. (a1) The enlarged image showing an unichromosomal SatS5 signal (red); (a2) The enlarged image showing two SatS5 signals (red) and two SatS3 signals (green), with one exceptionally weak SatS3 signal (green, hollow arrowhead) on a homologous chromosome; (a3) The enlarged image showing a SatS5 (red) signal on one of the synapsed chromosomes; (a4) The enlarged image showing an unichromosomal SatS5 signal (red, arrow head); (a5) The enlarged image showing a strong SatS3 signal (green, arrowhead) on one homologous chromosome and a faint one (hollow arrowhead) on the other partner; (a6) The enlarged image showing imbalanced SatS5 (red) and SatS3 (green) signals on a set of paired chromosomes, bar = 5 μm.