Figure 8

Model of p63 binding at distal regulatory elements of keratin-14 and RFX5. Histograms in green show p63 tag density in a 1 kb window at the regulatory region. Loops links the distal regulatory regions to their target promoter(s) as mapped by the ENCODE project [53]. (A) 2 p63 binding sites 64.3 kb and 380.5 kb upstream are linked to keratin-14 (KRT14). These regions also regulate other genes, among them keratin-16 (KRT16) and keratin-17 (KRT17). Motif analysis reveals other TFs binding within the 1 kb window. (B) p63 binds 469.4 kb upstream to RFX5 at a distal regulatory element linked to RFX5 promoter. This region is also linked to TUFT1 and RORC promoters. Motif analysis shows p63 binding in complex with AP1, E-box binding factors and BACH1 on a weak canonical motif. Additional motifs are also found within the 1 kb window.