Figure 4

Comparative analysis of E. coli O145:H28 with other EHEC strains. BLAST comparison of complete EHEC chromosomes against EcO145 str. RM13514 chromosome, using BLASTP with a cutoff value of 75% identity. Genomes are ordered from inner to outer ring: (1) E. coli O145:H28 str. RM13516 (yellow); (2-6) E. coli O157:H7 strains EC4115, TW14359, Sakai, EDL933, and Xuzhou21(light green); (7-9) non-O157 STEC strains 11368 (O26:H11), 12009 (O103:H2), and 11128 (O111:H-) (blue); (10) E. coli O145:H28 str. RM13514 mobile elements [Prophage–Navy, Prophage-like–Orange, Shiga toxin phage–Red, Integrated element–Green, LEE – pink]; (11-16) O-islands present in O157 str. EDL933, O145 str. RM13514, O145 str. RM13516, O26 str. 11368 (14), O103 str. 12009, and O111 str. 11128 (grey); (17) RM13514 negative strand CDS (lime); and (18) RM13514 positive strand CDS (green). The circular plot was generated using BLAST Ring Image Generator (BRIG) software[41]. EHEC core genome genes and serotype unique genes compared to O145 strains are in Dataset S2, and list of O-islands present in non-O157 genomes are in Additional file5: Dataset S3.