Table 6 PTEN deletion detection
From: Target enrichment using parallel nanoliter quantitative PCR amplification
Amplicon | Chip | Sample name | End point fluorescence | Cq | Mean coverage | Min coverage | Max coverage | Coverage stdev |
|---|---|---|---|---|---|---|---|---|
PTEN-4 | chip1 | Normal1 | 3409.9 | 19.9 | 766.1 | 66 | 1232 | 314.0 |
PTEN-4 | chip1 | Normal1 | 3219.8 | 20.1 | 494.6 | 40 | 800 | 204.5 |
PTEN-4 | chip1 | MCF7 | 3592.8 | 23.7 | 450.0 | 37 | 720 | 176.9 |
PTEN-4 | chip1 | MCF7 | 3204.2 | 23.2 | 520.2 | 42 | 879 | 216.8 |
PTEN-4 | chip2 | 786-O | 5000.1 | 22.4 | 732.0 | 57 | 1224 | 310.2 |
PTEN-4 | chip2 | HCT-15 | 4097.8 | 19.8 | 486.7 | 33 | 823 | 201.9 |
PTEN-4 | chip2 | MDA-MB-231 | 4508.4 | 19.2 | 386.4 | 29 | 643 | 159.5 |
PTEN-4 | chip2 | OVCAR-5 | 4188.0 | 19.9 | 282.8 | 19 | 452 | 107.8 |
PTEN-4 | chip3 | MOLT-4 | 3499.5 | 19.6 | 683.9 | 48 | 1094 | 272.9 |
PTEN-4 | chip3 | DU-145 | 4686.3 | 18.7 | 364.0 | 24 | 610 | 144.1 |
PTEN-4 | chip3 | SN12C | 3725.0 | 19.0 | 234.9 | 23 | 388 | 90.1 |
PTEN-4 | chip3 | BT-549 | 4036.6 | 20.0 | 422.5 | 45 | 643 | 150.1 |
PTEN-4 | chip4 | IGROV-1 | 4000.3 | 20.0 | 409.0 | 35 | 626 | 141.1 |
PTEN-4 | chip4 | CCRF-CEM | 0.0 | 40.0 | 0.4 | 0 | 2 | 0.7 |
PTEN-4 | chip4 | PC3 | 0.0 | 40.0 | 0.4 | 0 | 1 | 0.5 |
PTEN-4 | chip4 | HCT116 | 3906.9 | 20.1 | 627.4 | 62 | 1033 | 246.2 |
PTEN-4 | chip5 | Normal1 A | 4424.1 | 20.2 | 611.7 | 58 | 984 | 245.1 |
PTEN-4 | chip5 | ACHN | 3996.0 | 20.2 | 426.6 | 28 | 699 | 176.5 |
PTEN-4 | chip5 | HT-29 | 4239.3 | 20.2 | 256.0 | 26 | 396 | 90.6 |
PTEN-4 | chip5 | MCF7 | 4035.4 | 20.5 | 503.0 | 38 | 796 | 196.7 |
PTEN-4 | chip6 | Normal1 | 2955.7 | 20.5 | 602.4 | 39 | 1015 | 261.2 |
PTEN-4 | chip6 | Normal2 | 3482.0 | 20.7 | 378.8 | 33 | 613 | 145.4 |
PTEN-4 | chip6 | Normal2 | 3440.0 | 21.1 | 225.6 | 18 | 344 | 83.2 |
PTEN-4 | chip6 | RPMI-8226 | 3660.0 | 21.9 | 365.8 | 36 | 571 | 135.1 |