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Table 7 SNP detection in technical replicates

From: Target enrichment using parallel nanoliter quantitative PCR amplification

Sample

Capture reaction

PCR replicates

Total variants

Total variants (union)

Variants in comon in between all replicates (intersect)

Genotype concordant

Genotype discordant

Normal1 DNA

Duplicate-A

2

145

168 (15 indles)

120 (10 indels)

118 (10 indels)

2

 

Duplicate-B

2

133

    
 

Singelton-A

1

141

    
 

Singleton-B

1

143

    

MCF7

Duplicate-A

2

76

86 (16 indels)

72 (10 indels)

69 (10 indels)

3

 

Duplicate-B

2

77

    
 

Singelton

1

84

    

Normal2 DNA

Singelton-A

1

134

149 (14 indels)

121 (11 indels)

119 (11 indels)

2

 

Singleton-B

1

133

    
  1. The number of consistently and inconsistently called SNP positions across the technical replicates included in this experiment.