Figure 5

Local expression of immune genes in fin by qPCR. Local tissue expression was profiled in all three species at six days post exposure. Genes displayed in (A) were selected based on microarray analysis, and in (B) were selected based on previous analyses. As per the microarray analysis, all genes were normalized within a species and therefore the only valid comparison to make is between control (white) and infected (grey) within a species. In all three species, interferon response factor 7 (irf7) was down-regulated. Pink salmon up-regulated thioredoxin (txn) and down-regulated galectin 3-binding protein (lgals3bp), and Atlantic salmon up-regulated complement C7 (c7). Both Atlantic and chum salmon up-regulated prostaglandin D synthase (pgds), and pink salmon was the only species to induce pro-inflammatory cytokine interleukin-1 beta (il-1b), and slightly tumor necrosis factor alpha (tnf-a), although the fold change was low (1.3-fold). Boxplot displays median and interquartile range, and circles are outliers. *denotes p < 0.05; **denotes p < 0.001.