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Table 1 Identification, expression pattern, and functional characterization of differentially secreted proteins in Cochliobolus lunatus at different temperature regimes

From: Secretome weaponries of Cochliobolus lunatusinteracting with potato leaf at different temperature regimes reveal a CL[xxxx]LHM - motif

Spot #

aDiff exp.

Protein hits

bTh. p I/cExp. pI

dTh. Mr/eEx. Mr. / fNet Char.

gM. Sc.

hSeq. Cover.

Putative function

Interaction conditions

1

Up-regulated

123093|e_gw1.16.937.1

5.7/6.5

39.6/18.2/-4

182

49

Protein kinase activity

No potato leaf at 20°C

2

Up-regulated

16455|fgenesh1_pg.2_#_314

9.7/6.5

64.0/15.6/14

179

56

Modulate transcription

3

Up-regulated

118437|e_gw1.13.184.1

10.7/6.6

18.8/11.87/18

398

45

Unknown

4

Up-regulated

125544|e_gw1.19.437.1

7.2/5.5

58.7/42.4/4

365

46

Oxido-reductase activity

 

5

Up-regulated

24606|fgenesh1_pg.19_#_39

10.2/5.6

110.2/45.4/46

169

43

Protein kinase activity

6

Up-regulated

19257|fgenesh1_pg.7_#_229

6.2/5.8

195.0/46.3/-1.5

299

33

Unknown

7

Up-regulated

115068|e_gw1.10.331.1

4.2/6.0

261.8/45.2/-157

294

23

Heat shock protein 70kD

8

Up-regulated

129342|estExt_Genewise1.C_1_t20028

6.7/6.2

195.7/36.4/9

179

38

RNA binding binding protein

9

Up-regulated

115068|e_gw1.10.331.1

4.5/5.3

261.8/35.1/-157

279

31

HSP70

10

Up-regulated

129342|estExt_Genewise1.C_1_t20028

6.7/5.4

195.7/32.3/9

110

43

RNA processing

11

Up-regulated

19962|fgenesh1_pg.8_#_384

6.3/5.9

181.8/31.5/1

189

29

Protein kinase activity

12

Up-regulated

116559|e_gw1.11.1289.1

6.7/5.6

114.9/24.3/7.5

275

25

Subtilisin, proteolysis

13

Up-regulated

21721|fgenesh1_pg.12_#_162

9/5.6

146.7/21.4/24

161

26

Pleckstrin-like, intracellular signalling

14

Up-regulated

22022|fgenesh1_pg.12_#_463

10.4/6.8

27.5/16.0/18.5

382

32

Ribonuclease activity

15

Up-regulated

116337|e_gw1.11.1021.1

10.1/6.1

183.2/22.8/59

497

40

Gelsolin-like intratrafficking protein

16

Up-regulated

51954|fgenesh1_pm.10_#_434

10.1/6.1

63.9/16.0/20.5

340

40

DNA binding protein

17

Up-regulated

140405|estExt_Genewise1.C_210317

6.2/6.3

91.9/12.8/91.9

245

28

Arrestin-like signaling protein

18

Up-regulated

137106|estExt_Genewise1.C_13_t10496

4.9/5.9

126.1/14.53/-31.5

198

32

Tetratricopeptide intracellular trafficking

19

Up-regulated

46252|estExt_Genemark1.C_210087

10.0/5.7

52.3/12.3/20.5

287

22

Unknown

21

Up-regulated

64448|estExt_fgenesh1_pg.C_160063

9.9/5.3

112.8/13.1/53

111

29

Unknown

22

Up-regulated

59412|estExt_fgenesh1_pm.C_130013

7.2/5.3

109.9/13.4/12.5

100

34

WD40 repeat, signal transduction

23

Up-regulated

29439|fgenesh1_kg.13_#_117_#_Contig_2

7.1/5.0

28.6/10.7/3

276

39

Short-chain dehydrogenase/reductase

24

Up-regulated

58690|estExt_fgenesh1_pm.C_9_t10419

5.2/5.4

88.5/11.3/-14

369

21

Proteolysis

25

Up-regulated

125654|e_gw1.19.739.1

5.7/5.0

145.5/13.4/-9.5

476

24

Signal transduction response regulator

27

Up-regulated

132047|estExt_Genewise1.C_4_t40098

9.4/4.6

44.7/71.0/6.5

282

49

Unknown

Potato leaf at 20°C

28

Induced

123093|e_gw1.16.937.1

5.7/4.4

39.6/18.3/-4

165

21

Protein kinase activity

29

Induced

16455|fgenesh1_pg.2_#_314

9.7/4.2

64.0/14.1/14

186

38

DNA binding transcription factor

30

Induced

114571|e_gw1.10.1404.1

6.6/6.5

49.0/29.0/4

188

22

Carbohydrate metabolism

31

Induced

141118|estExt_Genewise1Plus.C_1_t20192

4.7/6.5

89.9/35.3/-26.5

359

28

Nucleic acid metabolism

32

Induced

44339|estExt_Genemark1.C_10_t10268

5.9/4.5

106.7/20.63/-6.5

162

29

Carbohydrate metabolism

38

Induced

46026|estExt_Genemark1.C_180273

11.2/6.2

21.8/18.6/1.5

195

31

Cytochrome C oxidase

Potato leaf at 38°C

  1. #Spot number corresponds to the spots in Additional file 3: Figure S1, Figures 5 and 6. aDifferential protein expression, bTheoretical isoelectric point (pI), cExperimental pI, dTheoretical molecular mass based on whole protein sequence, eExperimental molecular mass of protein subunits in KDa, fPredicted protein net charge based on whole protein sequence, gProtein score reported by MASCOT MS Ion search, and hAmino acid sequence coverage. This group of proteins was used for motif searching by in silico tools. All the proteins were identified by homology search at http://genome.jgi.doe.gov/Coclu2/Coclu2.home.html.