Table 3 Statistics of the first generation BAC-based physical map of half-smooth tongue sole genome

Number of BAC clones fingerprinted

33, 575

~8.3 × genome coverage

Valid fingerprints for FPC assembly

30, 294

~7.5 × genome coverage

Total number of contigs assembled

1,485

Clones contained in the 1,485 contigs

29,709

~7.3 × genome coverage

Average number of clones per contig

20

Average contig size in consensus bands (CB)

278

Estimated average contig size (kb)

539

Longest contig (ctg31; kb)

3,481

Estimated N50 contig size (kb)

664

Number of Q-contigs

101

6.8%

Number of Q-clones

133

0.45%

Number of singletons

585

1.93%

Average insert size of the BAC library (kb)

155

Bands number of per BAC clone

80.2

Average size each band represents (kb)

1.933

Total number of bands included in the contigs

412,292

Total physical length of assembled contigs (kb)

796,960

~1.27 × genome coverage