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Table 3 Summary of the horseradish peroxidase isoenzymes and associated data produced during this study

From: Peroxidase gene discovery from the horseradish transcriptome

Contig number

Name (* novel)

Length nt

GC content%

Accession #

CAI calculated

Intron #

Signal sequence length

Disulfide bridges (EDBCP prediction)

pI mature protein

-

C1A

1062

43.69

HE963800

0.812

3

30

[11–91] [44-49] [97–301] [177–209]

5.59

15901

C1B

1056

43.94

HE963801

0.808

3

28

[11–91] [44-49] [97–301] [177–209]

5.84

25148

C1C*

1059

45.14

HE963802

0.809

3

29

[11–91] [44-49] [97–301] [177–209]

6.49

25148_2

C1D*

1059

45.04

HE963803

0.810

3

29

[11–91] [44-49] [97–301] [177–209]

7.04

04627

C2

1044

42.91

HE963804

0.800

3

24

[11–91] [44-49] [97–301] [177–209]

8.56

-

C3

1050

46.76

HE963805

0.781

3

29

[11–91] [44-49] [97–300] [177–209]

7.71

Manual assembly

A2A*

1011

46.79

HE963806

0.761

3

31

[11–91] [44-49] [97–299] [176–208]

4.93

Manual assembly

A2B*

1011

46.69

HE963807

0.761

3

31

[11–91] [44-49] [97–299] [176–208]

4.93

04382

E5

1044

46.07

HE963808

0.771

3

27

[11–91] [44-49] [97–300] [177–209]

8.84

01805

1805*

1065

44.41

HE963809

0.797

3

31

[11–91] [44-49] [97–301] [177–209]

5.97

22684

22684.1*

1050

46.76

HE963810

0.770

3

29

[11–91] [44-49] [97–300] [177–209]

6.98

22684_2

22684.2*

1050

46.67

HE963811

0.772

3

29

[11–91] [44-49] [97–300] [177–209]

6.37

01350

1350*

975

50.97

HE963812

0.707

3

28

[11–91] [44-49] [97–292] [176–201]

8.67

02021

2021*

996

46.08

HE963813

0.788

3

29

[11–89] [44-49] [95–297]

9.46

23190

23190.1*

1080

49.26

HE963817

0.724

2

31 42

[11–92] [44-49] [98–293] [178–205] or [22–103] [55-60] [109–304] [189–216]

8.40 6.58

23190_2

23190.2*

1080

49.17

HE963817

0.722

2

31 42

[11–92] [44-49] [98–293] [178–205] or [22–103] [55-60] [109–304] [189–216]

8.60 7.09

04663

4663*

1077

47.82

HE963814

0.748

3

31

[11–91] [44-49] [97–299] [176–208]

4.48

06351

6351*

945

43.39

HE963816

0.786

3

18

[17–96] [50-55] [102–292] [180–206]

6.37

03523

3523*

960

44.58

HE963820

0.761

0

22

[11–92] [44-49] [98–293] [177–203]

8.99

05508

5508.1*

966

49.28

HE963815

0.735

2

24 30

[11-87] [44-49] [93–287] [171–198] or [17–93] [50-55] [99–293] [177–204]

8.49 8.47

05508_2

5508.2*

966

49.38

HE963815

0.735

2

24 30

[11-87] [44-49] [93–287] [171–198] or [17–93] [50-55] [99–293] [177–204]

8.49 8.47

22489_1

22489.1*

978

51.02

HE963818

0.726

2

23 34

[22–98] [55-60] [104–298] [182–209] or [11-87] [44-49] [93–287] [171–198]

8.93 8.51

22489_2

22489.2*

978

50.82

HE963819

0.727

2

23 34

[22–98] [55-60] [104–298] [182–209] or [11-87] [44-49] [93–287] [171–198]

8.93 8.51

06117

6117*

1008

47.02

HE963821

0.802

3

22 32

[11–91] [44-49] [97–298] [176–208] or [21–101] [54-59] [107–308] [186–218]

5.52 6.16

17517_1

17517.1*

972

48.46

HE963822

0.737

2

23 24

[12–88] [45-50] [94–296] [171–203] or [11-87] [44-49] [93–295] [170–202]

9.49 9.39

17517_2

17517.2*

972

48.56

HE963823

0.739

2

23 24

[12–88] [45-50] [94–296] [171–203] or [11-87] [44-49] [93–295] [170–202]

9.52 9.41

08562_1

08562.1*

996

47.09

HE963824

0.779

3

22 28

[11–91] [44-49] [97–298] [176–208] or [17–97] [50-55] [103–304] [182–214]

9.01 9.03

08562_4

08562.2*

996

47.79

HE963825

0.788

3

22 28

[11–91] [44-49] [97–298] [176–208] or [17–97] [50-55] [103–304] [182–214]

9.00 9.02

  1. The nucleotide sequences of 28 isoenzymes were submitted to EMBL. “*” indicates novel. “CAI” = codon adaptation index. If signal sequence predictions gave more than one alternative result, both signal sequence lengths are shown, separated by “/”. Disulfide bridges were predicted using both alternatives of the mature protein (EDBCP = Ensemble-based Disulfide Bonding Connectivity Pattern prediction server).