Peroxidase gene discovery from the horseradish transcriptome

BMC Genomics

Table 4 Summary of isoenzyme expression and characterization

Contig number	Name (* previously unknown)	Activity ABTS	Activity TMB	Activity guaiacol	Activity pyrogallol
-	C1A	+	+	+	+
15901	C1B	-	+	-	-
25148	C1C*	+	+	(+)	+
25148_2	C1D*	+	+	(+)	+
04627	C2	+	+	+	+
-	C3	+	+	+	+
Manual assembly	A2A*	+	+	+	+
Manual assembly	A2B*	+	+	(+)	(+)
04382	E5	+	+	+	+
01805	01805* (B1?)	+	+	-	-
22684	22684.1* (B2A?)	+	+	-	-
22684_2	22684.2* (B2B?)	+	+	-	-
01350	01350*	+	+	-	-
02021	02021*	-	-	-	-
23190	23190.1*	-	-	-	-
23190_2	23190.2*	n.d.	n.d.	n.d.	n.d.
04663	04663.1*	+	(+)	-	-
06351	06351*	+	+	+	+
03523	03523*	-	-	-	-
05508	05508.1*	+	+	+	+
05508_2	05508.2*	n.d.	n.d.	n.d.	n.d.
22489_1	22489.1*	+	+	(+)	+
22489_2	22489.2*	+	+	(+)	+
06117	06117*	-	-	-	-
17517_1	17517.1*	+	-	-	-
17517_2	17517.2*	+	+	+	+
08562_1	08562.1*	+	+	-	-
08562_4	08562.2*	+	+	-	+

Isoenzymes showing obvious peroxidase activity with the assay used are marked with “+”. Isoenzymes showing very low but detectable peroxidase activity with the assay used are marked with “(+)”. Isoenzymes with no activity detected during an observation period of 2 h are marked with “-“. Allelic variants not produced heterologously are marked with n.d. (no data available). Isoenzymes discovered during this study are marked with “*”.

ISSN: 1471-2164