Table 5 Nucleotide sequences of forward and reverse primers of two randomly chosen candidate genes for processing quality (pre-α/β-gliadin and γ-Gliadin) and one control (ADP ribosylation factor, ARF) used for their validations of differential expression between good and poor quality varieties using quantitative real time PCR (qRT-PCR)
Gene | Forward primer (5′-3′) | Tm (°C) | Reverse primer (5′-3′) | Tm (°C) | Product size (bp) |
|---|---|---|---|---|---|
ADP ribosylation factor (ARF) | TGATAGGGAACGTGTTGTTGAGGC | 57.4 | AGCCAGTCAAGACCCTCGTACAAC | 59.1 | 200 |
Pre-α/β-gliadin | GACCTTTCTCATCCTTGTCCTCCT | 57.4 | CTGTGAATATGGTAGTTGCGGCTG | 57.4 | 265 |
γ-Gliadin | TCTCTACAACAACAGATGAACCCCTG | 58.0 | GCCTTGTTGTTGTTCTTGCTGCATG | 57.7 | 225 |