Figure 10
From: BisQC: an operational pipeline for multiplexed bisulfite sequencing
A method to validate the inclusion of duplicate reads for coverage statistics. Individual reads where no duplicates are present (A) and where duplicates are included (B). Duplicates can be used in coverage statistics if the proportion of methylation in the duplicate read pool is significantly correlated to the non-duplicate read pool. In the locus shown here, there are four sites that are differentially methylated and the proportion of methylation in the non-duplicate and duplicate pools are similar.