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Table 4 Pooling and spike-in design for the analysis of the effects of non bisulfite-converted DNA pooled with bisulfite-converted DNA

From: BisQC: an operational pipeline for multiplexed bisulfite sequencing

Ā 

Lane

1

2

3

4

5

6

7

8

# of libraries

4*

4*

4*

4*

4*

1

6

12

Non bisulfite-converted DNA proportion

1%

10%

20%

30%

50%

20%

20%

20%

Ā 

1

Ā Ā Ā Ā Ā Ā 

B1

B1

2

A2

A2

A2

A2

A2

Ā 

A2

A2

3

Ā Ā Ā Ā Ā Ā Ā 

C3

4

Ā Ā Ā Ā Ā Ā Ā 

E4

5

Ā Ā Ā Ā Ā Ā Ā 

F5

Index #

6

Ā Ā Ā Ā Ā Ā Ā 

A6

7

D7

D7

D7

D7

D7

Ā 

D7

D7

Ā 

8

E8

E8

E8

E8

E8

Ā 

E8

E8

9

Ā Ā Ā Ā Ā Ā 

H9

H9

10

Ā Ā Ā Ā Ā Ā Ā 

C10

11

Ā Ā Ā Ā Ā Ā Ā 

D11

12

G12

G12

G12

G12

G12

G12

G12

G12

  1. *The same 4 libraries were used in lanes 1ā€“5, 7 and 8.