Figure 2

RIP-chip experiments with Exo2 and Eti1 proteins. (A) Box plots of the distribution of enrichments for RIP-chip experiments with Exo2 and Eti1. The y axis shows normalised log₂ enrichment ratios in the indicated RIP-chip experiments. White circles represent mRNAs that were not reproducibly enriched, while grey circles correspond to transcripts that consistently associated with the proteins. Exo2 interacts with only two mRNAs (eti1 and ppo1), while Eti1 does not associate with any transcripts. (B) Enrichment levels of eti1 and ppo1 mRNAs in Exo2 RIP-chips after cells were treated with puromycin or extracts were incubated with EDTA. The results for two independent biological replicates are shown. Both treatments disrupt the interactions between Exo2 and the transcripts, indicating that the interactions are cotranslational.