Figure 2

Quantification by Western blot of the concentration of LasR (A) and RhlR (B) when different P. aeruginosa strains were cultivated at 30°C and 37°C. Strains analyzed are: PAO1 (P), IGB83 (IG), M10 (M), ID4365 (ID) and 148. In panel A PAOR1 (ΔlasR::Tc) was used as negative control and is denoted by -. C: corresponds to the expression of LasR from pECP64 (A) and RhlR from pECP61.5 (B). MW refers to protein molecular weight standards. Lanes in B are divides by vertical lines to denote that the picture that is shown has been reconstructed because those lanes corresponding to PAO1 and IGB83 were not contiguous, and in the case of M10, the picture showing both lanes were exposed for a longer period than that where samples of strains ID4365 and 148 are shown.