Figure 1

A. Hbs-1 in the IT93K-503-1 x CB46 population. The heat-induced browning of seed coats locus, Hbs-1, was identified using datasets from two experiments. Hbs-1 spanned 60.09 cM to 60.53 cM on linkage group 8. SNP markers 1_0032 and 1_1128 were the most significant markers in the locus and are highlighted in red on the linkage group. However, 1_1128 had the highest association with the heat-induced browning phenotype, accounting for 62.8% and 77.3% of the phenotypic variance. The significance threshold is indicated by the horizontal broken line. B. Hbs-2 in the IT93K-503-1 x CB46 population. The heat-induced browning of seed coats locus, Hbs-2, was identified using datasets from two experiments. Hbs-2 spanned 36.82 cM to 51.79 cM on linkage group 3. SNP marker 1_1342 accounted for the highest percent phenotypic variance of 9.5% (LOD 2.11) and 12.3% (LOD 2.77) and is highlighted in red on the linkage group. The significance threshold is indicated by the horizontal broken line.