Figure 4

Comparison of the raw ratio data between MLPA, PRT and real-time QPCR using concordance plots. A. MLPA and PRT based measurements clearly demonstrates grouping around integer values and excellent correlation, as indicated by the R² value of 0.91. Five samples represent a difference between PRT and MLPA of a single copy, however clustering both PRT and MLPA measurements allows copy number to be unambiguously assigned. B. A comparison between MLPA and real-time QPCR demonstrates a greater degree of variation between the two measurements. An almost vertical spread of measurements on the concordance plot demonstrates a poor degree of clustering in the 3–6 copy range between the two assays. The correlation between real-time QPCR and MLPA is high (R² 0.87), suggesting a low level of precision with respect to QPCR.