Table 1 List of 31 S . sclerotiorum effector candidates selected based on their annotation

SS1G_00642

Chitin binding protein

Chitin recognition protein (PF00187.14)

563

SS1G_02014

Chitin binding protein

Chitin binding domain (PF03067)

426

SS1G_04786

Chitin binding protein

Chitin recognition protein (PF00187.14)

399

SS1G_09392

Starch binding domain containing protein

Chitin binding domain (PF03067)

398

SS1G_12336

Chitin binding protein

Chitin binding domain (PF03067)

294

SS1G_14184

Agglutinin isolectin 3-like

Chitin recognition protein (PF00187.14)

245

SS1G_12509

LysM domain protein

LysM (PF01476)

447

SS1G_07836

Concanavalin A lectin glucanase

Peptidase_A4 (PF01828)

252

SS1G_08698

Ricin-type toxin

Ricin-type beta-trefoil lectin domain (PF00652)

409

SS1G_01593

Serine protease inhibitor

Peptidase inhibitor I9 (PF05922)

95

SS1G_03282

Serine protease inhibitor

Peptidase inhibitor I9 (PF05922)

522

SS1G_12605

Alkaline serine protease alp1

Peptidase inhibitor I9 (PF05922)

400

SS1G_03611

Cystein-rich protein

CFEM (PF05730)

119

SS1G_13935

Cystein-rich protein

CFEM (PF05730)

529

SPEPs containing nuclear localization signal (NLS)

Protein ID

Annotation

PFAM domain(s)

Length

Comments

SS1G_01866

Ring-7 protein

PHD-like zinc-binding domain (PF13771); Ring finger domain (PF13639)

425

NLS (118–141)

SS1G_03146

-

-

193

NLS (147–155)

SS1G_04309

-

-

177

NLS (82–87)

SS1G_05895

Zinc finger CCCH-type domain containing protein

Zinc finger C-x8-C-x5-C-x3-H type (PF00642)

310

NLS (256–291)

SS1G_05938

-

-

212

NLS (143–150)

SS1G_06787

-

-

430

NLS (189–250; 340–349)

SS1G_06890

-

-

284

NLS (66–120; 143–167; 225–245)

SS1G_07404

-

DUF3108 (PF11306)

282

NLS (65–79; 118–138; 223–233)

SS1G_09050

-

-

454

NLS (410–413)

SS1G_11108

Ribosomal protein s17

-

373

NLS (338–348)

SS1G_13142

-

-

131

NLS (89–99)

SPEPs showing homology to fungal effectors or fungal effector candidates

Protein ID

Annotation

PFAM domain(s)

Length

Comments

SS1G_03080

SsNEP1

Necrosis inducing protein (PF05630)

246

Homolog to B. cinerea NEP1 (Staats et al., 2007); P. Sojae NIP (Qutob et al., 2002); studied in (Bashi et al., 2011)

SS1G_11912

SsNEP2

Necrosis inducing protein (PF05630)

245

Homolog to B. cinerea NEP1 (Staats et al., 2007); P. Sojae NIP (Qutob et al., 2002); studied in (Bashi et al., 2011)

SS1G_00849

AltA-1 allergen analog

-

152

Homolog to C. hingginsianum HE651255_CHEC91 Kleemann et al., 2012)

SS1G_02904

CVNH protein

CyanoVirin-N Homology domain (PF08881)

169

Homolog to C. hingginsianum HE651243_CHEC80 Kleemann et al., 2012)

SS1G_08858

Deuterolysin metalloprotease

Deuterolysin metalloprotease (M35) family (PF02102)

354

Homolog to M. oryzae AvrPita (MGG_15370 - Orbach et al., 2000)

SS1G_10096

Cerato-platanin (PF07249)

137

Homolog to B. cinerea BcSpl1 (Frias et al., 2011); H. atroviridis EPL1 (Seidl et al., 2006); M. oryzae MSP1 Jeong et al., 2007); C. hinginsianum HE651160_CHEC5 Kleemann et al., 2012)