Figure 3

Co-transcriptional cleavage of Leishmania spp ribozymes. The cladogram for the Leishmania SIDER2 syntenic insertion is shown in (A). The branch support values are given in %. The RNA constructs assayed are shown in (B). The dotted line indicates the cleavage point and the striped boxes indicate the ribozyme region. The result of co-transcriptional cleavage reactions is shown in (C). Note that the central panel is an overexposed version of the left panel LmexSIDER2A(83)Rz (line 3). The quantification of independent triplicates is shown in (D). Empty arrowheads indicate uncleaved products; solid arrowheads indicate the cleavage 3′ fragments. The empty circles indicate the cleavage 5′ fragments.