Figure 5

Cleavage kinetics of trypanosomatid HDV-like ribozymes. The uncleaved products of the transcription showed in Figure 3C (central panel) and 4D were gel-purified and subjected to cleavage reaction. Time course reactions at different Mg²⁺ concentrations were performed. Li/LdSIDER2A(81)Rz, LmSIDER2A(81)Rz, TvSIDER1(94)Rz and L1/NARTco(78)Rz kinetic reactions at 0.1 mM Mg²⁺ are shown in (A). The LmexSIDER2A(83)Rz kinetic reaction at 10 mM Mg²⁺ is shown in (B). The time course (min) of the reaction is shown above each line; the empty arrowheads indicate uncleaved products; solid arrowheads indicate cleavage 3′ fragments; the empty circle indicates the cleavage 5′-fragment. All the ribozyme reactions fitted the two-phase decay kinetic curve shown in (C); the plotted data are the results of independent assays performed in triplicate. A more extended study is shown in Additional file 1: Figure S3.