Skip to main content
Figure 2 | BMC Genomics

Figure 2

From: DNA-encoded nucleosome occupancy is associated with transcription levels in the human malaria parasite Plasmodium falciparum

Figure 2

Nucleosome positioning analysis across the erythrocytic cell cycle. A. The total number of nucleosomes in intergenic and coding regions as detected in our old MAINE-Seq data set (left panel) [18] compared to the total number of nucleosomes reported by PuFFIN using our new high-coverage MNase ChIP-Seq data set (right panel). B. Average scores of nucleosomes located in intergenic and coding regions at different time points during the asexual and sexual stages of the erythrocytic cell cycle. Values are expressed as the percentage of the average score of nucleosomes located inside genes at the ring stage. C. Average genome-wide sequence read coverage around the translation start site for a previously published nucleosome occupancy data set [20]) and the MNase ChIP-Seq data set from this study. Both data sets show a well-positioned first nucleosome inside the coding region, as well as reduced coverage in intergenic regions as compared to coding regions. Coverage is expressed as the fraction of the highest coverage value among all positions within the plot window. D. Nucleosomal organization in and around coding regions. The location and scores of nucleosomes detected by PuFFIN are shown for all 2,262 P. falciparum genes that do not contain any introns. Each line represents one gene, sorted from small (top) to large (bottom) and centered around its midpoint. More strongly positioned nucleosomes are represented by increasingly warmer colors. Troph.: trophozoite; schiz.: schizont; gam.: gametocyte.

Back to article page