Figure 5

Normalized expression profiles of genes modulated by selected transcription factors in response to carolacton treatment. (A) The normalized expression profiles of the genes co-regulated by the pyrimidine biosynthesis regulatory protein in the carolacton treatment context TRRN of S. mutans. Two operons containing genes encoding enzymes involved in the biosynthesis of pyrimidine ribonucleotides were upregulated sharply by about 1.8 to 2 log2-fold 5 min post carolacton treatment. (B) The expression dynamics of the genes co-regulated by the glutamine repressor GlnR in response to carolacton treatment. (C) The normalized expression profiles of the genes commonly regulated by the downregulated essential TCS response regulator vicR in response to carolacton. VicR modulated genes include those encoding virulence attributing products such as glucosyltransferases B and D, cell wall protein WapE among others. (D) Temporal behavior of the 26 genes found within the co-regulated group/subnetwork commonly modulated by SMU.852 encoding the CysR cysteine metabolism regulatory protein. Co-regulated gene groups were constructed by overlaying predicted regulator-binding site maps onto the co-expression network as shown here (E) specifically for the network confined to cysR. The node corresponding to the lone regulator (SMU.1509 encoding a putative Rgg family transcription factor) in the gene group co-regulated by CysR is marked in grey.