Dramatic expansion of the black widow toxin arsenal uncovered by multi-tissue transcriptomics and venom proteomics

Table 4 Predicted neurotoxin proteins identified in venom

ID	Best BLAST hit	# unique peptides	% coverage
Contig14^a	CRISP	42	51.90%
Contig29^b	CRISP	5	6.87%
LHV_229_Sp6	CRISP	5	24.30%
LHV_312_Sp6	CRISP	2	3.77%
Contig12556	CRISP	21	38.90%
Contig7836	CRISP	25	44.30%
venom_comp104578_c0_seq1	ICK	2	27.00%
HV209_Sp6_4	α-latrotoxin	2	11.60%
gi\|380876959\|sp\|P0DJE7.1\|LITD_LATHE	δ-latroinsectotoxin	3	17.00%
Contig2826	α-latroinsectotoxin	47	35.40%
Contig9724	α-latrotoxin	23	23.50%
Contig9749	α-latrotoxin	43	40.30%
Venom_comp106397_c0_seq1	δ-latroinsectotoxin	32	30.50%
Venom_comp107842_c0_seq1	δ-latroinsectotoxin	15	30.00%
Venom_comp107842_c2_seq1	δ-latroinsectotoxin	3	37.70%
Venom_comp109612_c1_seq1	δ-latroinsectotoxin	4	19.30%
Venom_comp110241_c0_seq1	α-latrotoxin	7	8.98%
Venom_comp110271_c0_seq3	α-latrotoxin	2	6.88%
Venom_comp110271_c1_seq1	α-latrotoxin	28	28.00%
Venom_comp110278_c1_seq1	α-latrotoxin	25	26.60%
Venom_comp110315_c0_seq1	α-latrotoxin	8	25.80%
Venom_comp110315_c1_seq1	α-latrotoxin	14	20.20%
Venom_comp110343_c2_seq1	δ-latroinsectotoxin	3	5.50%
Venom_Contig10080	δ-latroinsectotoxin	8	8.76%
Venom_Contig10081	δ-latroinsectotoxin	4	4.40%
Venom_Contig10082	δ-latroinsectotoxin	4	4.42%
Venom_Contig10158	δ-latroinsectotoxin	6	13.30%
Venom_Contig11531	α-latrocrustotoxin	44	35.90%

^aContains EST sequences LHV_167_Sp6, LHV_012_T7, LHV_012_Sp6, LHV_194_Sp6, LHV_167_T7.
^bContains EST sequences LHV_250_Sp6, LHV_144_Sp6, LHV_069_Sp6, LHV_069_T7, LHV_511_Sp6, LHV_123_T7, LHV_123_Sp6, LHV_144_T7, LHV_457_Sp6.
The type of toxin identified by the top BLAST hit to each transcript is shown in column 2. Proteomic evidence is displayed in columns 3 (unique peptides mapping to sequence) and 4 (% coverage of predicted protein with mapped peptides).

ISSN: 1471-2164