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Table 4 Unigene naming criteria are as follows

From: A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing

Method

 

Assignment

BDBH

 

Ortholog

BLASTp search

  

Alignment length

identity

 

> 85%

> 35%

High confidence

> 70%

> 25%

Homolog

< 70%

> 30%

Conserved domain

< 70%

< 30%

Low confidence

  1. If a gene is bi-directional best hit (BDBH) of X in A. thaliana (or P. trichocarpa), we term it ortholog of X; if it is similar to X in A. thaliana (or P. trichocarpa) using BLASTp and it aligns in 85% of its length with more than 35% identity, we term it a High confidence X in Q. suber, etc.