Evaluation of high throughput gene expression platforms using a genomic biomarker signature for prediction of skin sensitization

Table 5 Comparison of the number of detected genes in the 38 samples shared between all platforms

Gene symbol	nCounter®	BioMark HD™	OpenArray®
ABCB4	1 (3%)	31 (82%)	18 (47%)
FDXR	D	37 (97%)	36 (95%)
OR5B21	0 (0%)	D	37 (97%)
ADAM20	11 (29%)	D	35 (92%)
HIST1H3J	36 (95%)	D	36 (95%)
GDF11	30 (79%)	D	D
LY96	D	D	36 (95%)
TLR6	36 (95%)	D	D
CD86	33 (87%)	D	36 (95%)
FASN	D	D	37 (97%)
PFAS	D	D	36 (95%)
MAPK13	37 (97%)	D	D
GDAP2	D	D	36 (95%)
TMEM97	35 (92%)	D	D
DHX33	35 (92%)	D	D
RFC2	D	D	37 (97%)
ACLY	D	D	35 (92%)
TXNRD1	D	D	37 (97%)
HPRT1	D	D	37 (97%)
GAPDH	D	D	37 (97%)

The set of 33 transcripts were measured in the 38 chemical stimulations shared between all platforms. The table illustrates the number of detected calls for a specific transcript across the 38 samples with percentage in parentheses. Table lists only transcripts declared as undetected in any of the stimulations on either nCounter®, BioMark HD™ or OpenArray®. D denotes that transcript is present in all samples.

ISSN: 1471-2164