Figure 3

Neighbor-Joining tree summarizing the relationships among the alpha subunits of the dioxygenases of strains PP1Y, F199 and US6-1. Colours indicate the localization of the ORFs: blue PP1Y/chromosome; green, PP1Y/megaplasmid; red, F199/chromosome; magenta, F199/pNL1; brown, F199/pNL2; black, US6-1/chromosome; gray, US6-1/pLA1. The numbers following the name of the oxygenases refer to the gi accession numbers of the NCBI protein database. The analysis involved 164 amino acid sequences (the sequences used to prepare the tree in Additional file 1: Figure S4 plus 18 sequences from strain US6-1). All positions containing gaps were eliminated. There was a total of 150 positions in the final dataset. For clarity all the branches not containing sequences from strains PP1Y, F199 and US6-1 were compressed.