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Table 6 GO enrichment analysis of the most variable genes in granulosa cells from small healthy follicles using GOEAST†

From: Transcriptome profiling of granulosa cells from bovine ovarian follicles during atresia

Biological process

Molecular function

Cellular component

Symbol

Entrez gene name

Regulation of vascularity Angiogenesis (P = 0.009)

  

CYR61

cysteine-rich angiogenic inducer

COL18A1

collagen 18

THBS1

thrombospondin 1

CXCR4

chemokine receptor 4

ANGPT2

angiopoietin 2

VEGFA

vascular endothelial growth factor A

Energy metabolism Glycogen biosynthetic process (P = 0.044)

Insulin-like growth factor binding, two component sensor activity

microsome

ISR2

insulin receptor 2

EGFR

epidermal growth factor receptor

HSP90AA1

heat shock protein 90 kDa alpha (cytosolic), class A member 1

PDK4

pyruvate dehydrogenase kinase, isozyme 4

IGFBP2

insulin-like growth factor binding protein 2

CTGF

connective tissue growth factor

CYR61

cysteine-rich angiogenic inducer

MGST1

glutathione transferase 1

CALR

calreticulin

PTGES

prostaglandin E synthase

PLC4

phospholipase C, beta 4

OPTN

optineurin

ASPSCR1

alveolar soft part sarcoma chromosome region, candidate 1

Cell Cycle Mitosis (P = 2.3 × 10-5)

 

Condensed chromosome kinetochore, spindle

CCNA2

cyclin A2

CCNB1

cyclin B1

CCNB2

cyclin B2

UBE2

ubiquitin conjugating enzyme 2

CDK1

cyclin-dependent kinase 1

NUSAP

nucleolar and spindle associated protein 1

SKA1

spindle and kinetochore associated complex subunit 1

NUP85

nucleoporin 85 kDa

CENPN

centromere protein N

CENPO

centromere protein O

ZW10

kinetochore associated homolog

BUB1

budding inhibited by benzimidazoles 1 homolog (yeast)

STK

serine/threonine kinase

Extracellular matrix Extracellular matrix organisation (P = 0.01)

Heparin binding, extracellular matrix binding

Basement membrane

COL1A1

collagen type I, alpha 1

COL1A2

collagen type I, alpha 2

COL3A1

collagen type III, alpha 1

COL4A3

collagen type IV, alpha 3

COL4A4

collagen type IV, alpha 4

COL18

collagen type XVIII

LAMB1

laminin, beta 1

VCAN

versican

FMOD

fibromodulin

ADAMTS1

ADAM metallopeptidase with thrombospondin type 1 motif, 1

ADAMTS6

ADAM metallopeptidase with thrombospondin type 1 motif, 6

MMP2

matrix metallopeptidase 2

TIMP1

TIMP metallopeptidase inhibitor 1

OGN

osteoinductive factor/osteoglycin

OSF-2

osteoblast-specific factor 2

SPARCL1

SPARC-like 1 (hevin)

ASPN

asporin

Inflammation

 

Fibrinogen complex

THBS1

thrombospondin 1

NOS biosynthesis (P = 0.044)

CALR

calreticulin

Antigen processing (P = 0.026)

BOLA-A

major histocompatibility complex (MHC), class I, A

TGFβ receptor signalling pathway (P = 0.05)

BOLA-N

MHC class I antigen

Fibrinolysis (P = 0.09)

Cell migration

  

CXCR4

chemokine receptor 4

Axon guidance (P = 0.0008)

ABLIM1

actin binding LIM protein 1

COL18

collagen type XVIII

EFNA5

ephrin A5

THBS1

thrombospondin 1

EGFR

epidermal growth factor receptor

TDGF

teratocarcinoma-derived growth factor 1

F2RL1

coagulation factor II (thrombin) receptor-like 1

INSR

insulin receptor

MAPK activity (P = 0.002)

  

THBS1

thrombospondin 1

TDGF

teratocarcinoma-derived growth factor 1

   

INSR

insulin receptor

  1. †The significance of association for the GO enrichment analysis was determined by the Benjamini-Yekuteli FDR method for multiple comparisons. The variability of expression was determined by a frequency distribution of the coefficients of variation for probe sets across the arrays in the small healthy follicle group. The cut-off chosen was a coefficient of variation of > 46.8% (n = 10 arrays, n = 682 probe sets).