Figure 3

Gene transfer of rps14 . A. Schematic diagram of rps14 gene transfer from mitochondrial genome to the nucleus. The mitochondrial rps14 copies are identified in two repeat regions and are co-transcribed with rpl5. Boxes indicate mitochondrial targeting presequence (mTP; blue) and a conserved domain (ribosomal S14; red). The grey box arrow in the nuclear genome represents the non-functional copy of rps14 and the dotted red line indicates a conserved domain. Internal stop codons are indicated with asterisks. B. Nucleotide sequence alignment of the two nuclear, transcript, and mitochondrial copies of rps14 from Rhazya stricta. Shaded red box shows an 8 bp deletion that caused a frameshift. C. Amino acid sequence alignment of two nuclear and one mitochondrial copy of rps14 of Rhazya with seven nuclear-encoded and five mitochondrial copies from other angiosperms (see Additional file 1: Table S13). Blue boxes indicate mitochondrial targeting presequences. Red boxes indicate the conserved domain of ribosomal S14. mt = mitochondrial, n = nuclear.