Figure 2

Localization of miRNA in the chromosome as polycistronic cluster and expression of trophectoderm/placenta specific/imprinted miRNAs in different stages of embryos and placentas from different sources of pregnancies. A: Genomic region (44 kb) of bovine chromosome 21 harboring at least 3 big clusters of miRNAs which are down regulated in day-50 NT and IVP placentas compared to that of AI. Text in blue above the black line (forward genomic strand) represents the residing miRNAs; number in pink represents range and scale of the region in kilo bases (kb), number in black starting with rs- denotes dbSNPs, others are different types of transposone (red or orange) namely type I line/SINE, type II, tandem repeats (trf) and pseudo transfer RNA (tRNA). B-E: Expression pattern (in fold change) of the candidate trophectoderm/placenta specific (B) and imprinted miRNAs (C) in blastocyst, expanded blastocyst, day-16 elongated embryo, day-50 placenta and day-225 placentome derived from IVP pregnancies compared to that from AI. Expression pattern (in fold change) of the candidate trophectoderm/placenta specific (D) and imprinted miRNAs (E) in blastocyst, expanded blastocyst, day-16 elongated embryo, day-50 placenta and day-225 placentome derived from NT pregnancies compared to that of AI pregnancies. F: Methylation status of AGO2 promoter region in the day-50 placentas from different sources of pregnancies. Note the degree of methylation in NT placentas significantly differed from AI placentas.