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Figure 5 | BMC Genomics

Figure 5

From: Massive deregulation of miRNAs from nuclear reprogramming errors during trophoblast differentiation for placentogenesis in cloned pregnancy

Figure 5

Derivation, culture, validation and knocking down of AGO2 during differentiation of trophoblastic stem cells (TBSs). Culture of blastocyst at day 12 (A), derivation of TBSs at day 14 of culture (B), Fully grown TBSs (C and F), TBSs plated for subculture (D), developing trophoblastic vesicles (VSC) (E), differentiated TBSs into distinctive placental cells (G), nontransfected differentiated TBSs (H) and differentiated TBSs transfected with AGO2 siRNA (I). J: Expression of Homeobox protein CDX-2, Interferon tau-(ifn-tau), pregnancy-associated glycoproteins 2-PAG2 and Glyceraldehyde 3-phosphate dehydrogenase-GAPDH mRNAs (as control) in TBSs (TB), nontransfected differentiated TBSs (DTBN) and differentiated TBSs transfected with AGO2 siRNA (DTBSi). Blue arrow indicates binucleate cells and green arrow shows trophoblastic giant cells.

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BMC Genomics

ISSN: 1471-2164

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