Figure 1

Overview of gene expression profiling approach used. A) Images of control and Pax3^Spd/Spd mutant humeri at Theiler stage 23 dissected prior to RNA extraction (right hand image); the images on the left are external views of stage and genotype matched 3D scanned specimens stained to reveal the morphology and ossification more clearly. White arrow heads indicate the elbow joint and shoulder joint line and red arrow heads the ossification site, a visible reduction is apparent in the mutant compared to the control in each case. B) Work-flow of Microarray and RNA-sequencing data analysis.