Identification of mechanosensitive genes during skeletal development: alteration of genes associated with cytoskeletal rearrangement and cell signalling pathways

Table 6 Differentially expressed genes associated with other signalling pathways

Down-regulated			Up-regulated
Bone morphogenetic protein signalling pathway
Fold change		Gene symbol	Gene title	Role in pathway
RNA-seq	Microarray	Gene symbol	Gene title		Reference
87.94	50.88	Hfe2	Hemochromatosis type 2	Co-Rec	[59]
5.25		Fgf8	Fibroblast growth factor 8	T	[60]
2.44		Scx	Scleraxis	T	[61]
2.17		Egr2	Early growth response 2	T	[62]
2.03		Bmp3	Bone morphogenetic protein 3	L
Hedgehog signalling pathway
14.55	4.59	Hhatl	Hedgehog acyltransferase-like	L-act	[63]
2.70		Ptchd1	Patched domain containing 1	R
2.27		Hhip	Hedgehog interacting protein	Ant, T	[64]
2.14		Ptch1	Patched 1	T	[65]
Fibroblast growth factor signalling pathway
#		Fgf4	Fibroblast growth factor 4	L
#		Fgf6	Fibroblast growth factor 5	L
5.25		Fgf8	Fibroblast growth factor 8	L
5.10		Fgf5	Fibroblast growth factor 5	L
4.54	3.23	Fgfr4	Fibroblast growth factor receptor 4	R
3.24		Spry1	Sprouty homolog 1	Ant	[66]
3.22		Spry4	Sprouty homolog 4	Ant
2.75		Spry2	Sprouty homolog 2	Ant	[66]
2.39		Fgf2	Fibroblast growth factor 2	L
2.134	2.48	Fgf10	Fibroblast growth factor 10	L
Hippo signalling pathway
98.29	49.05	Vgll2	Vestigial like 2 homolog (Drosophila)	Co-TF	[67]
3.86	4.38	Tead4	TEA domain family member 4	Co-TF	[68]
2.96		Fat4	Fat tumor suppressor homolog 4	R	[69]
2.43		Fat2	Fat tumor suppressor homolog 2	R	[69]
Notch signalling pathway
5.64		Dner	Delta/notch-like EGF-related receptor	R	[70]
3.16	2.21	Dll1	Delta-like 1 (Drosophila)	L	[71]
2.69		Hes6	Hairy and enhancer of split 6	T	[72]
2.54		Dtx4	Deltex homolog 4	T	[72]
2.09		Jag2	Jagged 2	R	[71]
3.25	2.87	Nrg1	Neuregulin 1	T	[73]
2.36	2.09	Foxc2	Forkhead box C2	Upstream-activator	[74]
2.27	2.29	Cntn1	Contactin 1	PL	[75]

#No numerical value for fold change as no transcripts detected in the Mutant.
Co-Rec -Co-Receptor, T -Target, L -Ligand, L-act- Ligand activator, R -Receptor, Ant -Antagonist, Co-TF- -Co-Transcription Factor, PL –Potential Ligand.

ISSN: 1471-2164