Figure 1

Glycerol utilization and transcriptional regulation of GOA1 and NDH51 by the TRKOs. A. Drop plate assays of 6 TRKO mutants in YP-dextrose (YPD) or YP-glycerol (YPG). Each mutant was spot inoculated with 3 μl of stock cells and grown at 30°C for 24 h. X- and Y-sets refer to two independently constructed mutants lacking each gene. The reduction or lack of growth on YPG indicates a mitochondrial respiratory dysfunction. B. Each of the mutants shown in (A) was measured for transcription of GOA1 by Real-time PCR. Data are indicated as a down regulation of either GOA1 or NDH51. The latter encodes a Complex I subunit of the mitochondrial electron transport chain. Dpb4p, Hfl1p and Rbf1p are positive regulators of both GOA1 and NDH51.