Figure 2

The yeast to hyphal transition, generation time, and transporter activity are altered in each of the TRKO mutants. A. Growth on Spider agar, YPD pH 9.5 and YPD pH 5.0. On Spider agar, all mutants had reduced colony growth. On YPD (below), colony diameter was again reduced and each mutant had a wrinkled colony appearance with a lack of filamentation at pH 9.5. B. Microscopic growth of each mutant is shown. In YPD (30°C), each of the TRKO mutants was constitutively filamentous compared to the yeast growth of WT cells (SN250) as reported previously [21, 22]. In 10% serum, all mutants and WT strains are filamentous. C. Doubling times are shown for WT (SN250) as well as each TRKO strain. Strains dpb4∆ and rbf1∆ were significantly higher than WT cells. Strain hfl1∆ was not significantly different than WT cells. Doubling time was determined by cell mass measurements.