Figure 5

Cytokine expression from primary monocyte-derived macrophages. CD14+ cells were obtained from six donors and allowed to mature into macrophages by incubation for five days. The cells were then infected with the three isolates, Maa 1794, Mah 1655 and Mah VI101, at a MOI of 10:1 for 24 hours or left uninfected (neg). The supernatant was removed and assessed for IL-6, IL-8, IL-10, IL-23 and TNF-α by the Bio-plex assay. The six donors are represented with individual symbols. Each point is the median of triplicate wells. The horizontal line represents the mean response of the six donors. Mah VI101, consistently produced more IL-8, IL-23 and TNF-α than Maa 1794 and more IL-23 than Mah 1655. The difference was significant (p < 0.05) using the non-parametric Wilcoxon signed rank test.