Figure 2

Integer CN assignment using a clustering algorithm for (A) PRT4 and (B) MLPA. Results of a typical 96-well plate/batch are shown for PRT4 and MLPA, respectively. The dark blue lines indicate the means of the clusters with their values above and assigned integer CNs in brackets. The light blue lines indicate the primary cluster center obtained from reference samples. Circles with the same color represent one cluster. The dashed line indicates the threshold of likelihood below which samples were discarded.