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Table 1 Long-range PCR primers for amplifying the complete human mitochondrial genome

From: From cheek swabs to consensus sequences: an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes

Primer name

Sequence (5′–3′)

Length (nt)

5′–3′ binding position in revised Cambridge Reference Sequence (rCRS)

Expected product size (bp)

HumLR_1F

ACGGGAAACAGCAGTGATTAAC

22

807–828

8511

HumLR_1R

CTAGTATGAGGAGCGTTATGGAGTG

25

9342–9318

HumLR_2F

GTACGCCTAACCGCTAACATTACT

24

8998–9021

8735

HumLR_2R

GTTTTAAGCTGTGGCTCGTAGTG

23

1163–1141