Skip to main content

Table 2 Sequences and conditions for new primers used in SYBR Green real-time RT-PCR assays

From: Metabolic consequences of microRNA-122 inhibition in rainbow trout, Oncorhynchus mykiss

Gene

Forward primer (5′3′)

Reverse primer (3′5′)

Tm (°C)

Gene bank or SIGENAE accession number

Amplicon size (bp)

omy-miRNA-122

TGGAGTGTGACAATGGTGTTTGT

Poly T Primer (Invitrogen)

60

-

-

cyp2k5

CTCACACCACCAGCCAAGAC

CGTCAGCAGAGGTAACACATCAG

60

NM_001124742

76

ptgr1

CAGTGATTGTGGATGGAGGAG

CCTTGATCTTGGCGATCTGT

60

NM_001124634

238

arcn1

AGCCTCACTTGTGGGAGAGA

CTTGTCACCGTTGTTGATGG

60

NM_001164069

119

Gs

AAGATATGGAGGCGGAGAGG

GATCTCAAGGACCAGGGTTG

60

BX299627.p.om.8

152

Pygl

AACCGACACCTCCACTTCACC

CCTGCATCTTCCTCCATCTC

60

BX882218.p.om.8

296

srebp2

TAGGCCCCAAAGGGATAAG

TCAGACACGACGAGCACAA

60

BX859204.p.om.8

161

Hmgcs

AGTGGCAAAGAGAGGGTGTG

TTCTGGTTGGAGACGAGGAG

60

CX251971.p.om.8

298

Hmgcr

GAACGGTGAATGTGCTGTGT

GACCATTTGGGAGCTTGTGT

60

AB218825.p.om.8

216

dhcr7

GTAACCCACCAGACCCAAGA

CCTCTCCTATGCAGCCAAAC

60

CA376644.p.om.8

289

cyp7a

ACGTCCGAGTGGCTAAAGAG

GGTCAAAGTGGAGCATCTGG

60

AB675933

111

cyp7b

ACAGAGACCTCACCTTCACCA

GATCTCCCTTCCTCACTCCA

60

F6VG15V01BFI5C.p.om.8

171

abcg5

CACCGACATGGAGACAGAAA

GACAGATGGAAGGGGATGAA

60

CU073172.p.om.8

268

abcg8

GATACCAGGGTTCCAGAGCA

CCAGAAACAGAGGGACCAGA

60

FYV3OTN01BVHON.p.om.8

159

utg1a3

CCACCAGCAAGACAGTCTCA

CAACAGCACAGTGGCTGACT

60

F6VG15V01BF6CZ.p.om.8

216

  1. Gene bank accession number (http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/genbank) are provided when available. For the remaining sequences, accession numbers from the trout EST database SIGENAE (http://www.sigenae.org) are provided. Additional primer sequences have been previously published and can be found in sources cited in the text.