Figure 11

Wnt7 splice variants in Edwardsiella and Nematostella. A. Amino acid alignment of Wnt7A and 7B transcripts from E. lineata and N. vectensis. In the gray region, the amino acid sequence and the underlying nucleotide sequence of E. lineata Wnt7A is identical to that of E. lineata Wnt7B. Similarly, the amino acid sequence and underlying nucleotide of N. vectensis Wnt7A is identical to that of N. vectensis Wnt7B. In the regions of the alignment highlighted in blue and pink, the amino acid sequence of E. lineata Wnt7A is most similar to N. vectensis Wnt7A (blue) and the amino acid sequence of E. lineata Wnt7B is most similar to N. vectensis Wnt7B. B. A maximum likelihood phylogeny based on amino acid sequences of Wnt7A and 7B but excluding the portion of the alignment shared by E. lineata Wnt7A and Wnt7B (the region shaded in gray). Numbers at nodes indicate how many times the given clade was recovered in 1000 replications of the bootstrap. The scale bar represents the number of substitutions per site. Taxon abbreviations are as follows: El = Edwardsiella lineata; Hs = Homo sapiens; Nv = Nematostella vectensis. C. Diagram of the Nematostella Wnt7 locus illustrating the similarities and differences of the Wnt7A/7B splice variants (adapted from [66]). Wnt7A is composed of sequences from exons 1b, 2, 3, and 6, and Wnt7B is composed of exons 1, 1b, 2, 3, 4, and 5.