Figure 3

Unsupervised PCA of the granulosa cell arrays using Partek. The arrays are numbered (n = 4 per treatment) as follows: control granulosa (G1, G5, G9 and G13 in grey); FSH-treated (G2, G6, G10 and G14 in blue); TNFα-treated (G3, G7, G11 and G15 in red) and TNFα plus FSH-treated (G4, G8, G12, and G16 in black). The graph is a scatter plot of the values for the first (X) and second (Y) principal components based on the correlation matrix of the total normalised array intensity data. The groups of arrays (A) and (B), encircled by the dotted lines, represent related arrays based on patterns of total gene expression determined from the PCA. The G9 array had a considerably different pattern of gene expression from all the others and was therefore considered to be a statistical outlier and excluded from further analysis.