Figure 7

Correlation of gene expression ratios between the two replicates (A), and between sequencing data and quantitative RT-PCR data (B). (A) Reproducibility analysis of two independent libraries constructed from root tissue treated with CdCl₂ for 6 hours. The relativity analysis was based on the TPM from these two libraries. (B) Pearson correlation scatters plots of comparisons of ratios measured by sequencing and quantitative RT-PCR in mRNAs and miRNAs. Thirty genes, including 20 mRNAs and 10 miRNAs, were randomly selected and were subjected to quantitative real-time PCR analysis. The rice UBC and U6 RNAs were used as internal standards. Sequencing data (fold changes in gene expression) were plotted against qRT-PCR data (fold-changes in gene expression). Both the x and y-axes are shown in log₂ scale. r indicates the Pearson correlation coefficient.