Figure 3

Phylogenetic tree of WRKY domains between cotton and Arabidopsis . The amino acid sequences of the WRKY domain of cotton and Arabidopsis were aligned with MUSCLE, and the phylogenetic tree was constructed using the JTT model with an estimated γ-distribution parameter (G). The maximum-likelihood analyses were performed with the program PhyML version 3.0, and assessment of node confidence was performed using 1,000 bootstrap replicates. The members of group I, II (a-e) and III are labelled according to the classifications of AtWRKY domains by Eulgem et al. [33]. The triangles indicate cotton WRKY genes, and filled triangles represent the genes analysed by qPCR. Contig9787 was named GhWRKY70-like1; was named contig5359 GhWRKY64-like1; and contig16334 was named GhWRKY72-like1 after calculating the p-distance to determine the closest relationship with Arabidopsis members.