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Figure 5 | BMC Genomics

Figure 5

From: Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae

Figure 5

Comparison of expression levels by “ in situ ” qPCR of a subset of 32 DEG. These genes were examined from two different areas (stamen and carpel) isolated from 6 mm cotton flower buds infested by cotton boll weevil larvae by laser microdissection (LMD) in relation to the control. The reference genes GhACT4 and GhFBX6 were used to normalise the qPCR data. The relative expression level was calculated using the relative expression software tool (REST©), and a subsequent statistical test of the analysed CP values by a Pair-Wise Fixed Reallocation Randomization Test was performed.

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