Table 3 Virulence factors determined by CGH for NT clonal complexes

Competence proteins

comA; competence factor transporting ATP-binding/permease protein ComA

1

1

1

1

1

1

1

1

1

comB; competence factor transport protein ComB

1

1

1

1

1

1

1

1

1

comD; putative sensor histidine kinase ComD

1

1

1

1

1

1

1

1

1

comE; response regulator ComE

1

1

1

1

1

1

1

1

1

comX1; transcriptional regulator ComX1

1

1

1

1

1

1

1

1

1

competence damage-inducible protein A

1

1

1

1

1

1

1

1

1

coiA; competence protein CoiA

1

1

1

1

1

1

1

1

1

competence protein ComF, putative

1

1

1

1

1

1

1

1

1

celA; competence protein CelA

1

1

1

1

1

1

1

1

1

celB; competence protein CelB

1

1

1

1

1

1

1

1

1

ccs1; competence-induced protein Ccs1

1

1

1

1

1

1

1

1

1

ccs4; competence-induced protein Ccs4

1

1

1

1

1

1

1

1

1

ccs16; competence-induced protein Ccs16

1

1

1

1

1

1

1

1

1

cspC-related protein, authentic point mutation

1

1

1

1

1

1

1

1

1

pilD; type IV prepilin peptidase, putative

1

1

1

1

1

1

1

1

1

comGA/cglA; competence protein CglA

1

1

1

1

1

1

1

1

1

comGB/cglB; competence protein CglB

1

1

1

1

1

1

1

1

1

comGC/cglC; competence protein CglC

1

1

1

1

1

1

1

1

1

comGD/cglD; competence protein CglD

1

1

1

1

1

1

1

1

1

comGE

1

1

1

1

1

1

1

1

1

comGF

1

1

1

1

1

1

1

1

1

comGG

1

1

1

1

1

1

1

1

1

Choline-binding proteins

cbpA/pspC; choline binding protein A

0

0

0

0

0

0

0

0

0

cbpD; choline binding protein D

1

1

1

1

1

1

1

1

1

cbpE/pce; choline binding protein E

1

1

1

1

1

1

1

1

1

cbpF; choline binding protein F

0.1

0.9

1

0

1

0.3

0

0

1

cbpG; choline binding protein G

0.9

1

1

1

1

1

1

1

1

lytA; autolysin

1

1

1

1

1

1

1

1

1

lytB; endo-beta-N-acetylglucosaminidase

1

1

1

1

1

1

1

1

1

lytC; beta-N-acetylhexosaminidase

1

1

1

1

1

1

1

1

1

pspA; pneumococcal surface protein A

0

0

0

0

0

0

0

0

0

pcpA; choline binding protein PcpA

0.9

0.1

0

0

0

0

0

0

0

Colonisation-associated proteins

hyl; hyaluronidase

0.9

1

1

1

1

1

1

0.5

1

nanA; neuraminidase A/siliase A precursor

0.1

0

0

1

0

0

0

0.5

1

pavA; adherence and virulence protein A

1

1

1

1

1

1

1

1

1

rlrA; transcriptional regulator, putative

0

0

0

0

0

0

0

0

0

bgaA; beta-galactosidase

0.1

0.9

1

0

1

0.3

0

0

0

eno; phosphopyruvate hydratase

1

1

1

1

1

1

1

1

1

pyrR; bifunctional pyrimidine regulatory protein PyrR uracil phosphoribosyltransferase

1

1

1

1

1

1

1

1

1

strH; beta-N-acetylhexosaminidase

1

1

1

1

1

1

1

1

1

trpG; anthranilate synthase component II

1

1

1

1

1

1

1

1

1

phoU; phosphate transport system regulatory protein PhoU, putative

0.1

0.9

1

1

1

0.3

0

0.5

0

rr01; DNA-binding response regulator

1

1

1

1

1

1

1

1

1

transcriptional regulator SPY2053

1

1

1

1

1

1

1

1

1

Other major virulence factors

ply; pneumolysin

1

1

1

1

1

1

1

1

1

psaA; manganese ABC transporter, manganese-binding adhesion lipoprotein

1

1

1

1

1

1

1

1

1

htrA; serine protease

1

1

1

1

1

1

1

1

1

IgA; immunoglobulin A1 protease

1

1

1

1

1

1

1

1

1

spxB; pyruvate oxidase

1

1

1

1

1

1

1

1

1

piaA; iron-compound ABC transporter, iron compound-binding protein

0.1

0.9

0

0

0

0.3

0

0.5

1

piaB; iron-compound ABC transporter, permease protein

0.1

0.9

0

0

0

0.3

0

0.5

1

piaC; iron-compound ABC transporter, permease protein

0.1

0.9

0

0

0

0.3

0

0.5

1

piaD; iron-compound ABC transporter, ATP-binding protein

0.1

0.9

0

0

0

0.3

0

0.5

1

piuA; iron-compound ABC transporter, iron-compound-binding protein

1

1

1

1

1

1

1

1

0

piuB; iron-compound ABC transporter, permease protein

1

1

1

1

1

1

1

1

0

piuC; iron-compound ABC transporter, permease protein

1

1

1

1

1

1

1

1

0

piuD; iron-compound ABC transporter, ATP-binding protein

1

1

1

1

1

1

1

1

0

zmpB; zinc metalloprotease

0

0.8

0

0

0

0.3

0

0

1